IEEE Catalog Number : ISBN : CFP 12 NMD - PRT 978 - 1 - 4 2012 IEEE Nanotechnology Materials and Devices Conference ( NMDC 2012 )

In recent years, the biochip by MEMS technology has been developed. The biochip has the advantages of biocompatibility, precision, low-cost, disposable, and easy to produce etc. MEMS technology widely used in cell detection or position a single-cell level, including the mechanical, optical electromagnetic and electronic fields. This study proposal, imitate the oviduct that can effectively position the oocyte by dielectrophoresis microchannel chip technology. The biochip increases the opportunity of sperm in the natural fertilization of the collision, and directly replaces the traditional microinjection. The in vitro fertilization (IVF) is an important technique in biological and clinical studies. We propose the imitation oviduct microfluidic system to perform IVF on a chip. Imitation of IVF, the oocyte was positioned through the dielectrophoresis (DEP) force in the microchannel, and then collided with the pre-treatment of sperms driven by syringe pump to form the embryo, the purpose of natural fertilization on the chip. The collision frequency between the cells plays the success key role of the IVF. Therefore to position oocyte in the microchannel and flow motile sperms to make more collision would be very important for the formation of health embryo. The numerical simulation software, CFDRC, was used to reveal the position of high and low electric field distribution in microchannel. The position of oocyte and the sperm collide oocyte were successfully achieved to enhance the probability of natural fertilization when the 10 Vpp and 1 MHz frequency were applied.